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A实验方法是严新对分离出来的某肺癌标志性细胞进行发功,然后进行了PCR检测

Materials and methods

SCLC cell culture

NCI-H82 cell line was derived by Gazdar in 1978 from the pleural fluid of a 40-year-old patient with SCLC. This cell line has been well characterized and used as an in vitro model for studying lung cancer [15, 16]. Cells were cultured in 24-well plates with Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal calf serum and 1% Pen/Strep in a 37°C incubator with 5% CO2.

YXQ-EQ treatment

NCI-H82 cells were treated with YXQ-EQ as described previously [13].

外链出处

NIH对于文献搜索的收录也有一定的原则吧,不是什么乱七八糟都可以收录进去

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