主题：【原创】玉米种子 PH4CV 的专利说明书 -- 厚积薄发

claims

权利要求

1. A seed of maize inbred line designated PH4CV, representative seed of said line having been deposited under ATCC Accession No. PTA-4673.

一种玉米自交系，名为PH4CV，此种系已经提交美国典型物种保藏中心（ATCC），序号PTA-4673

2. A maize plant, or a part thereof, produced by growing the seed of claim 1.

由1中所述玉米种子得到的玉米植株以及植株的部分

11. A method of producing an herbicide resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers herbicide resistance.

一种通过向2中所述的玉米转入外源基因而得到抗除草剂特性的方法。

12. An herbicide resistant maize plant produced by the method of claim 11.

由11中所述方法得到的抗除草剂玉米

14. A method of producing an insect resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers insect resistance.

一种通过向2中所述的玉米转入外源基因而得到抗虫特性的方法。

15. An insect resistant maize plant produced by the method of claim 14.

由方法14得到的抗虫玉米

任何通过以下方法，向PH4CV导入性状的过程（都是本专利发明人的权益）

(a) crossing PH4CV plants grown from PH4CV seed, representative seed of which has been deposited under ATCC Accession No: PTA-4673, with plants of another maize line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of waxy starch, male sterility, herbicide resistance, insect resistance, bacterial disease resistance, fungal disease resistance, and viral disease resistance;

把由PH4CV种子得到的植株（ATCC PTA-4673号）和其它玉米的植株杂交得到杂交子一代F1。这些"其它玉米"带有想得到的性状，包括:蜡状淀粉（有咬劲），雄性不育，抗除草剂，抗虫，抗细菌病，抗真菌病，抗病毒。

(b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants;

选育带有某种性状的杂交子一代

(c) crossing the selected progeny plants with the PH4CV plants to produce backcross progeny plants;

把子一代和PH4CV回交

(d) selecting for backcross progeny plants that have the desired trait and the alleles of inbred line PH4CV at the SSR loci listed in Table 4 to produce selected backcross progeny plants; and

选择带有特定性状的回交产物，而且该产物带表4列出的SSR位点（大概是PH4CV的特征位点，用来鉴定该产物也是带了PH4CV的特性）

(e) repeating steps (c) and (d) to produce backcross progeny plants that comprise the desired trait and comprise at least 95% of the alleles of inbred line PH4CV at the SSR loci listed in Table 4.

重复c和d，直到得到的回交产物有特定的性状，而且在表4列出的的SSR位点上，有至少95％的自交PH4CV系的等位基因（保证得到的产物基本还是PH4CV，除了加了一点点其他玉米的成分）。